DISPERSION OF PLURIPOTENT STEM CELLS, AND PLURIPOTENT STEM CELL PRODUCT AND METHOD FOR PRODUCING SAME
|Inventor||KUWAHARA Atsushi, etc|
|Assignee||SUMITOMO DAINIPPON PHARMA CO., LTD. , etc.|
|Title of Invention||DISPERSION OF PLURIPOTENT STEM CELLS, AND PLURIPOTENT STEM CELL PRODUCT AND METHOD FOR PRODUCING SAME|
Provided is a method for producing a pluripotent stem cell product comprising steps of; (1) culturing pluripotent stem cells in an undifferentiated cell maintenance culture medium, (2) suspending the pluripotent stem cells cultured in (1) in a first cell suspension medium containing a ROCK inhibitor, (3) replacing the cell suspension medium in the suspension obtained in (2) by a cryopreservation medium to produce a cell dispersion composed of the cryopreservation medium and the pluripotent stem cells dispersed in the cryopreservation medium, and (4) filling the cell dispersion obtained in (3) in an air-tight container in an air-tight state.