Patent Portfolio
Method for genome editing
| Ref. No. | AJ178 |
|---|---|
| Appl. No. （filing date） |
PCT/IB2017/054736（2017/8/2） |
| Pub. No. （Pub. date） |
WO2018/025206（2018/2/8） |
| Priority claim No. （Priority date） |
US 62/370,047 (2016/08/02) |
| Patent No. | - |
| Inventor | WOLTJEN, Knut, etc. |
| Assignee | Kyoto University |
| Title of Invention | Method for genome editing |
| Abstract | Provided is a method for producing a cell having a scarless genome sequence wherein an exogenous nucleic acid sequence inserted into a targeted region in the genome is completely excised, wherein the exogenous nucleic acid sequence comprises a nucleic acid sequence homologous to a genome sequence in the targeted region at each end and one or more sequence-specific nuclease-recognizing site(s) between the two homologous nucleic acid sequences, and wherein the method comprises: (1) introducing the sequence-specific nuclease or a nucleic acid encoding the same into a host cell having a genome sequence into which the exogenous nucleic acid sequence is inserted; and (2) culturing the cell obtained in step (1), thereby causing double-strand break at the sequence-specific nuclease-recognizing site(s) and the subsequent microhomology-mediated end joining or single-strand annealing between the resulting broken ends that contain the homologous nucleic acid sequences to generate a cell having a scarlessly reverted genome sequence. |
| Comments |
- |
| Updated date | 2019/6/13 |
| Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2018-025206/27F93C22A278599EB688A25AFBF11D2DF6C3D3D2D10A697A698CFEF76C6F05B2/50/en |
| Tag | Genome Editing |