Patent Portfolio
High expression mRNA switch
| Ref. No. | AJ160 |
|---|---|
| Appl. No. （filing date） |
PCT/JP2018/026362（2018/7/12） |
| Pub. No. （Pub. date） |
WO2019/013294（2019/1/17） |
| Priority claim No. （Priority date） |
JP2017-136469 (2017/7/12) |
| Patent No. | - |
| Inventor | SAITO Hirohide, etc. |
| Assignee | Kyoto University |
| Title of Invention | High expression mRNA switch |
| Abstract | Provided is a mRNA comprising (i) a nucleic acid sequence specifically recognized by a miRNA or a protein, and (ii) a nucleic acid sequence of a coding region of a marker protein, wherein the mRNA contains N1-methyl-pseudouridine nucleotide. When introduced into a cell, the said mRNA functions as a mRNA switch, either on- or off-switch, to control the expressions of a marker protein in response to an intracellular miRNA or protein. The mRNA switch of the invention shows an elevated expression level when introduced into a cell, and hence in an embodiment, a large increase in the expression of a marker protein can be achieved relative to the prior art, especially for a membrane protein or a secretory protein. |
| Comments |
- |
| Updated date | 2019/2/18 |
| Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2019-013294/EC36BC6C48332E801A0211252379323DC63E31D7E02E112D56E3858C76276C64/50/en |