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Method for producing low-antigenic cell

Ref. No. AJ165
Appl. No. (filing date) PCT/JP2019/005524 ( 2019/02/15)
Pub. No. (Pub. date) WO2019/160077 (2019/08/22)
Priority claim No. (Priority date)

JP2018-026421 (2018/02/16)

Patent No.

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Inventor HOTTA Akitsu, etc.
Assignee Kyoto University
Title of Invention Method for producing low-antigenic cell
Abstract

Provided is a method for producing, from a donor cell, a low-antigenic cell that causes a reduced immune rejection when allogenically transplanted into a recipient, the method comprising a selective deletion of HLA alleles that are present in the donor cell but not in the recipient (donor-specific HLA alleles) through a targeted disruption or modification of the donor-specific HLA alleles by means of genome-editing technology. Being distinct from B2M knock-out cells (HLA-null cells), the low-antigenic cells of the invention are capable of evading NK cell attack (missing-self response) while having antigen-presenting ability. One aspect of the invention pertains to those cells in which both HLA-A alleles and HLA-B alleles are disrupted but at least one allele of HLA-C is retained (HLA-C retained cells). The HLA-C retained cells are especially useful in the field of cell therapy, since 12 kinds of HLA-C retained cells cover more than 93% of the world's population.(The low-antigenic cells of the invention are capable of evading NK cell attack (missing-self response) while having antigen-presenting ability. Especially the cells in which both HLA-A and HLA-B alleles are disrupted but HLA-C is retained (HLA-C retained cells) are useful in the field of cell therapy, since 12 kinds of HLA-C retained cells cover more than 93% of the world's population.)

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