Related Patent Info
GENERATION OF A POPULATION OF HINDBRAIN CELLS AND HINDBRAIN-LIKE ORGANOIDS FROM PLURIPOTENT STEM CELLS
| Inventor | BUTTS, Jessica C., etc. |
|---|---|
| Assignee | THE J. DAVID GLADSTONE INSTITUTES, etc. |
| Title of Invention | GENERATION OF A POPULATION OF HINDBRAIN CELLS AND HINDBRAIN-LIKE ORGANOIDS FROM PLURIPOTENT STEM CELLS |
| Abstract | Provided is a method for generating a 3D organoid comprising a population of hindbrain cells comprising a heterogeneous population of interneurons, the method comprising steps of treating a population of mammalian pluripotent stem cells (PSCs) in vitro with a WNT signaling pathway activator and culturing the population of PSCs in a neural induction medium comprising a retinoic acid signaling pathway activator, a sonic hedgehog signaling pathway activator and a Notch signaling pathway inhibitor. |