Related Patent Info
LUNG AND AIRWAY PROGENITORS GENERATED FROM HUMAN PLURIPOTENT STEM CELLS AND RELATED TREATMENTS
| Inventor | SNOECK, Hans-Willem, etc. |
|---|---|
| Assignee | THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
| Title of Invention | LUNG AND AIRWAY PROGENITORS GENERATED FROM HUMAN PLURIPOTENT STEM CELLS AND RELATED TREATMENTS |
| Abstract | Provided is a method for generating lung progenitor cells comprising steps of (a) producing anterior foregut endoderm cells from mammalian pluripotent stem cells (PSCs), (b) culturing the anterior foregut endoderm cells in a suspension culture to generate at least one lung bud organoid (LBO), (c) embedding the LBO within a 3D matrix, (d) culturing the embedded LBO to form branched LBO (BLBO), and (e) dissociating the LBO or BLBO and culturing the dissociated LBO or BLBO on feeder cells in a culture medium. |