Related Patent Info
METHOD OF DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MONOHORMONAL CELLS
| Inventor | CHEN, Shuibing, etc. |
|---|---|
| Assignee | CORNELL UNIVERSITY |
| Title of Invention | METHOD OF DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MONOHORMONAL CELLS |
| Abstract | Provided is a method for generating an enriched population of monohormonal cells from pluripotent stem cells comprising steps of exposing pluripotent stem cells sequentially to (a) CHIR99021 and Activin A, (b) Activin A, (c) FGF7, (d) retinoic acid, LDN 193189 and SANT 1, and (e) retinoic acid, LDN193189, SANT1, EGF and FGF2, wherein at least 50% of the cells in the generated population are monohormonal and express insulin, but not glucagon, somatostatin, or ghrelin. |