Related Patent Info

METHOD FOR DIFFERENTIATION OF OCULAR CELLS AND USE THEREOF

Inventor CHASE, Lucas, etc.
Assignee FUJIFILM CELLULAR DYNAMICS, INC.
Title of Invention METHOD FOR DIFFERENTIATION OF OCULAR CELLS AND USE THEREOF
Abstract

Provided is a method for producing a population of neural retinal progenitors (NRPs) comprising steps of; (a) obtaining a starting population of human iPSCs, (b) culturing the cells in a first retinal differentiation medium (RD1) comprising a BMP inhibitor to further differentiate the cells to anterior neuroectoderm cells, (c) inducing retinal differentiation of the anterior neuroectoderm cells by culturing the cells in a second retinal differentiation medium (RD2) essentially free of BMP inhibitors to form retinal progenitor cells (RPCs), and (d) culturing the RPCs in a retinal maturation (RM) medium to produce NRPs. Also provided is a method for producing a population of photoreceptor precursor cells (PRPs) from NRPs obtained by the above method by further culturing in photoreceptor precursor induction medium (FDSC) comprising a g-secretase inhibitor and FGF for a period of time sufficient to produce a population of PRPs.