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Provided is a method for preparing human pluripotent stem cell (hPSC)-derived functional cardiomyocytes through cell differentiation and maturation, comprising steps of (i) culturing hPSC in a Matrigel-coated culture medium, (ii) inducing the differentiation of hPSC in step (i) to mesoderm in a mesodermal induction medium containing insulin-free B27 and a wnt signaling activator, (iii) differentiating the cells in step (ii) in a differentiation medium containing insulin-free B27, (iv) inducing myocardial differentiation of the cells in step (iii) in a myocardial differentiation inducing medium containing insulin-free B27 and a wnt signaling regulator, and (v) maturing the differentiated cardiomyocytes in step (iv) into functional cardiomyocytes by culturing in a myocardial maturation medium containing vitamin A-free B27, IL-15, and NGF.