Related Patent Info
INDUCTION OF NEURAL PROGENITOR CELLS, OLIGODENDROCYTE PROGENITOR CELLS, AND OLIGODENDROCYTES BY STEM CELL DIFFERENTIATION USING LANDMARK TRANSCRIPTION FACTORS
| Inventor | WANG, Jiwu, etc. |
|---|---|
| Assignee | ALLELE BIOTECHNOLOGY AND PHARMACEUTICALS, INC. |
| Title of Invention | INDUCTION OF NEURAL PROGENITOR CELLS, OLIGODENDROCYTE PROGENITOR CELLS, AND OLIGODENDROCYTES BY STEM CELL DIFFERENTIATION USING LANDMARK TRANSCRIPTION FACTORS |
| Abstract | Provided is a method for inducing differentiation of iPSCs and/or ESCs (SCs) into neural progenitor cells (NPCs) comprising steps of (1) plating SCs on a coated, non-tissue treated, culturing vessel in medium, (2) transfecting the SCs with mRNA encoding one or more cell-fate factors (in a preferred embodiment, selected from the group consisting of Ngn2, Pax6, Sox2, Brn2 and FoxO family factors) as individual mRNA or combined mRNA, and (3) culturing said SCs of step (2) until the cells differentiate into NPCs. Further provided is a method for generating oligodendrocyte progenitor cells (OPCs) from NPCs comprising steps of (4) plating NPCs on coated cell culture vessels in medium, and (5) transfecting the cells of step (4) with mRNA encoding one or more cell-fate factors (in a preferred embodiment, selected from the group consisting of 01igo2, NKX2.2, SOX10, and Oligol) as individual mRNA or combined mRNA to differentiate NPCs into OPCs. |