Related Patent Info
METHOD FOR STEPWISE INDUCTION FROM HEPATOBLASTS INTO BILIARY EPITHELIAL PROGENITOR CELLS
| Inventor | OSAFUNE, Kenji, etc. |
|---|---|
| Assignee | KYOTO UNIVERSITY |
| Title of Invention | METHOD FOR STEPWISE INDUCTION FROM HEPATOBLASTS INTO BILIARY EPITHELIAL PROGENITOR CELLS |
| Abstract | Provided is a method for producing biliary epithelial progenitor cells comprising a step of culturing hepatoblasts which may be induced from human pluripotent stem cells, in a medium containing TGFβ and EGF. Further provided is a method for constructing a 3D duct-like structure of biliary epithelial progenitor cells comprising a step of culturing hepatoblasts or biliary epithelial progenitor cells in a medium containing HGF, EGF, a Notch inhibitor and a GSK3 inhibitor in the presence of a 3D scaffold. |