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METHODS OF PRODUCING VENOUS ANGIOBLASTS AND SINUSOIDAL ENDOTHELIAL CELL-LIKE CELLS AND COMPOSITIONS THEREOF

Inventor KELLER, Gordon, etc.
Assignee UNIVERSITY HEALTH NETWORK
Title of Invention METHODS OF PRODUCING VENOUS ANGIOBLASTS AND SINUSOIDAL ENDOTHELIAL CELL-LIKE CELLS AND COMPOSITIONS THEREOF
Abstract

Provided is a method for producing sinusoidal endothelial cell-like cells (SEC-LCs) comprising steps of providing stem cells, which may be iPS cells, or angioblasts, and culturing the stem cells or angioblasts under conditions comprising; a) culturing the stem cells or angioblasts in the presence of bFGF; or b) culturing the stem cells or angioblasts in the presence of VEGF-A to produce endothelial cells followed by culturing the endothelial cells in the presence of a TGF-beta signaling inhibitor, cAMP signaling agonist, VEGF-C; or c) culturing the stem cells or angioblasts under hypoxic conditions, thereby producing SEC-LCs.