Related Patent Info
PRIMED CARDIAC PROGENITORS AND METHODS FOR MAKING AND USING SAME
|BIERMANN, Mitch, James, etc.
|WISCONSIN ALUMNI RESEARCH FOUNDATION
|Title of Invention
|PRIMED CARDIAC PROGENITORS AND METHODS FOR MAKING AND USING SAME
Provided is a method for producing a population of primed cardiac progenitors from pluripotent stem cells, comprising steps of (i) activating Wnt/β-catenin signaling in cultured pluripotent stem cells to obtain a first cell population, (ii) culturing the first cell population until cardiac mesoderm cells are present in the cell population, and (iii) inhibiting Wnt/β-catenin signaling in the cardiac mesoderm cells in the presence of an activator of innate immunity until a second cell population comprising primed cardiac progenitors is produced. As a preferred embodiment, the said activator of innate immunity is a TLR 3 ligand or a NF-κB activator.