Related Patent Info
METHOD FOR OBTAINING MICROGLIA FROM PLURIPOTENT STEM CELLS
|SHIONOGI & CO., LTD.
|Title of Invention
|METHOD FOR OBTAINING MICROGLIA FROM PLURIPOTENT STEM CELLS
Provided is a method for efficiently producing microglia from pluripotent stem cells, comprising steps of (a) co-culturing a pluripotent stem cell with a feeder cell to obtain a blood progenitor cell, (b) co-culturing the obtained blood progenitor cell with a feeder cell in the presence of IL-3 and/or GM-CSF to obtain an embryonic monocyte, and (c) in the presence of M-CSF, co-culturing the obtained embryonic monocyte together with an astrocyte, or culturing the embryonic monocyte using an astrocyte supernatant.