Related Patent Info
IN VITRO METHODS OF DIFFERENTIATING STEM CELLS INTO NEURONS AND NEURONS GENERATED USING THE SAME
|BUTTS, Jessica, etc.
|THE J. DAVID GLADSTONE INSTITUTES, etc.
|Title of Invention
|IN VITRO METHODS OF DIFFERENTIATING STEM CELLS INTO NEURONS AND NEURONS GENERATED USING THE SAME
Provided is a method for generating spinal cord glutamatergic interneurons from a population of human pluripotent stem cells (hPSCs), comprising a step of culturing a first population of hPSCs in a neural induction medium comprising retinoic acid signaling pathway activator, a sonic hedgehog signaling pathway activator, and Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells comprising CHX10+ V2a interneurons.