Related Patent Info
IN VITRO METHODS OF DIFFERENTIATING STEM CELLS INTO NEURONS AND NEURONS GENERATED USING THE SAME
| Inventor | BUTTS, Jessica, etc. |
|---|---|
| Assignee | THE J. DAVID GLADSTONE INSTITUTES, etc. |
| Title of Invention | IN VITRO METHODS OF DIFFERENTIATING STEM CELLS INTO NEURONS AND NEURONS GENERATED USING THE SAME |
| Abstract | Provided is a method for generating spinal cord glutamatergic interneurons from a population of human pluripotent stem cells (hPSCs), comprising a step of culturing a first population of hPSCs in a neural induction medium comprising retinoic acid signaling pathway activator, a sonic hedgehog signaling pathway activator, and Notch signaling pathway inhibitor, wherein the culturing results in generation of a second population of cultured cells comprising CHX10+ V2a interneurons. |