Related Patent Info
A METHOD FOR CULTURING MYOGENIC CELLS, CULTURES OBTAINED THEREFROM, SCREENING METHODS, AND CELL CULTURE MEDIUM
|PIJNAPPEL, Wilhelmus Wenceslaus Matthias, etc.
|ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM
|Title of Invention
|A METHOD FOR CULTURING MYOGENIC CELLS, CULTURES OBTAINED THEREFROM, SCREENING METHODS, AND CELL CULTURE MEDIUM
Provided is a method for producing a population of expanded myogenic progenitor cells, comprising steps of (a) culturing pluripotent stem cells (PSCs) in a medium supporting differentiation towards a myogenic lineage in the presence of (i) CHIR99021 in a certain concentration range, (ii) FGF2 in a certain concentration range, and optionally (iii) insulin-transferrin-selenium-ethanolamine, to thereby provide a cell culture comprising myogenic progenitors cells, (b) isolating from said cell culture C-Met+ and HNK1- myogenic progenitor cell, and (c) expanding said isolated cell in a medium comprising FBS and FGF2, to thereby provide a cell culture wherein at least 50% of said population are MyoD positive and Pax7 negative.