Related Patent Info
METHODS OF PRODUCING VENOUS ANGIOBLASTS AND SINUSOIDAL ENDOTHELIAL CELL-LIKE CELLS AND COMPOSITIONS THEREOF
| Publication date | 2020/03/26 |
|---|---|
| Inventor | KELLER, Gordon, etc. |
| Assignee | UNIVERSITY HEALTH NETWORK |
| Title of Invention | METHODS OF PRODUCING VENOUS ANGIOBLASTS AND SINUSOIDAL ENDOTHELIAL CELL-LIKE CELLS AND COMPOSITIONS THEREOF |
| Abstract | Provided is a method for producing sinusoidal endothelial cell-like cells (SEC-LCs) comprising steps of providing stem cells, which may be iPS cells, or angioblasts, and culturing the stem cells or angioblasts under conditions comprising; a) culturing the stem cells or angioblasts in the presence of bFGF; or b) culturing the stem cells or angioblasts in the presence of VEGF-A to produce endothelial cells followed by culturing the endothelial cells in the presence of a TGF-beta signaling inhibitor, cAMP signaling agonist, VEGF-C; or c) culturing the stem cells or angioblasts under hypoxic conditions, thereby producing SEC-LCs. |