GENERATION OF PANCREATIC PROGENITOR CELLS AND BETA CELLS FROM HUMAN PLURIPOTENT STEM CELLS
|Inventor||LIAN, Xiaojun, etc.|
|Assignee||THE PENN STATE RESEARCH FOUNDATION|
|Title of Invention||GENERATION OF PANCREATIC PROGENITOR CELLS AND BETA CELLS FROM HUMAN PLURIPOTENT STEM CELLS|
Provided is a method for generating beta cells from pluripotent stem cells (PSCs) comprising steps of; (a) culturing PSCs in a medium comprising a GSKP inhibitor and a BMP inhibitor for 4-10 days to obtain definitive endoderm (DE) cells, (b) culturing DE cells in a medium comprising a GSKP inhibitor, a BMP inhibitor, and a Hedgehog inhibitor for 2-6 days to obtain pancreatic progenitor 1 (PP1) cells, (c) culturing PP1 cells in a medium comprising a BMP signaling inhibitor for 2-6 days to obtain pancreatic progenitor 2 (PP2) cells, (d) culturing PP2 cells in a medium comprising T3, and a Hedgehog inhibitor, a retinoic acid-signaling activator, a Notch inhibitor, and a TGF-beta inhibitor for 5-10 days to obtain endocrine progenitor (EP) cells, and (e) culturing EP cells in growth-factor-free, defined medium for 10-20 days to obtain beta cells.