STEM CELL-DERIVED CELL CULTURES, STEM CELL-DERIVED THREE-DIMENSIONAL TISSUE PRODUCTS, AND METHODS OF MAKING AND USING THE SAME
|Inventor||CANTO-SOLER, Maria Valeria, etc.|
|Assignee||THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, etc.|
|Title of Invention||STEM CELL-DERIVED CELL CULTURES, STEM CELL-DERIVED THREE-DIMENSIONAL TISSUE PRODUCTS, AND METHODS OF MAKING AND USING THE SAME|
Provided is a three-dimensional tissue product derived from human iPSCs comprising functionally matured retinal pigment epithelial (RPE) cells, a portion of three-dimensional neural retina (3DNR), an additional biocompatible component, and a biocompatible scaffold, wherein the 3DNR, the RPE cells, and the additional biocompatible component are physically and functionally integrated to form a complex containing a layer of neural retina and an underlying layer of RPE cells, wherein the 3DNR comprises (i) undifferentiated pseudostratified neural retinal epithelium, (ii) laminated neural retina tissue including all retinal layers and their corresponding retinal precursor cell types, or (iii) advanced differentiated retinal tissue including an outer nuclear layer (ONL) and a bipolar cell layer (BCL), wherein the ONL could be rod-enriched, cone- enriched, or any combination thereof, wherein the additional biocompatible component comprises a natural or synthetic compound in a liquid or gel form that provides an appropriate biomechanical environment for cell survival and function, allows manipulation of the product, or both, and wherein the RPE cells are grown on top of said biocompatible scaffold prior to integration with the 3DNR, and wherein the 3DNR is positioned on top of the RPE cells.