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LUNG AND AIRWAY PROGENITORS GENERATED FROM HUMAN PLURIPOTENT STEM CELLS AND RELATED TREATMENTS

Publication No. WO2019/217429
Publication date 2019/11/14
Patent No.

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Inventor SNOECK, Hans-Willem, etc.
Assignee THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Title of Invention LUNG AND AIRWAY PROGENITORS GENERATED FROM HUMAN PLURIPOTENT STEM CELLS AND RELATED TREATMENTS
Abstract

Provided is a method for generating lung progenitor cells comprising steps of (a) producing anterior foregut endoderm cells from mammalian pluripotent stem cells (PSCs), (b) culturing the anterior foregut endoderm cells in a suspension culture to generate at least one lung bud organoid (LBO), (c) embedding the LBO within a 3D matrix, (d) culturing the embedded LBO to form branched LBO (BLBO), and (e) dissociating the LBO or BLBO and culturing the dissociated LBO or BLBO on feeder cells in a culture medium.

Updated date 2019/12/26
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