Related Patent Info.

METHOD OF DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MONOHORMONAL CELLS

Publication No. WO2019/217875
Publication date 2019/11/14
Patent No.

-

Inventor CHEN, Shuibing, etc.
Assignee CORNELL UNIVERSITY
Title of Invention METHOD OF DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MONOHORMONAL CELLS
Abstract

Provided is a method for generating an enriched population of monohormonal cells from pluripotent stem cells comprising steps of exposing pluripotent stem cells sequentially to (a) CHIR99021 and Activin A, (b) Activin A, (c) FGF7, (d) retinoic acid, LDN 193189 and SANT 1, and (e) retinoic acid, LDN193189, SANT1, EGF and FGF2, wherein at least 50% of the cells in the generated population are monohormonal and express insulin, but not glucagon, somatostatin, or ghrelin.

Updated date 2019/12/26
Comments -
Back to top