METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO BRAIN MICROVASCULAR ENDOTHELIAL CELLS
|Inventor||MATSUNAGA Tamihide, etc.|
|Assignee||PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY|
|Title of Invention||METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO BRAIN MICROVASCULAR ENDOTHELIAL CELLS|
Provided is a method for inducing differentiation of pluripotent stem cells into cerebrovascular endothelial cells capable of forming tight junctions that are stable and can be maintained for a long period of time, comprising steps of; (1)cultivating pluripotent stem cells in the absence of FGF2 and reducing undifferentiation, and (2)differentiating the cells obtained in step (1) into brain microvascular endothelial cells, said step including cultivation in the presence of FGF2, retinoic acid and a TGF-β inhibitor.