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PRODUCTION METHOD FOR PANCREATIC β CELLS

Publication No. WO2019/208788
Publication date 2019/10/31
Patent No.

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Inventor IBUKI Masato, etc.
Assignee KANEKA CORPORATION, etc.
Title of Invention PRODUCTION METHOD FOR PANCREATIC β CELLS
Abstract

Provided is a method for producing pancreatic β cells, comprising steps of; (a) producing posterior foregut (PFG) cells by culturing primitive gut tube (PGT) cells derived from pluripotent stem cells, in the presence of a protein kinase C (PKC) activator, (b)producing pancreatic precursor (PP) cells from PFG by culturing in the presence of retinoic acid or an analog thereof, (c) producing pancreatic endocrine precursor (EP) cells from PP by culturing in the presence of a Notch signal inhibitor and a ROCK signal inhibitor (or in a medium containing nicotinamide), and (d) producing pancreatic β cells from EP by culturing in the presence of an insulin receptor signal activator, transferrin, and selenious acid.

Updated date 2019/12/04
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