Maintenance-and-amplification method and differentiation induction method for primordial germ cells/primordial germ cell-like cells
|Appl. No. (filing date)||PCT/JP2018/045011 (2018/11/30)|
|Pub. No. (Pub. date)||WO2019/107576 (2019/06/06)|
|Priority claim No. (Priority date)||
|Inventor||SAITOU, Mitinori, etc.|
|Title of Invention||Maintenance-and-amplification method and differentiation induction method for primordial germ cells/primordial germ cell-like cells|
Present invention provides a maintenance and amplification method of PGC/PGCLC comprising a step of culturing PGC/PGCLC in the presence of a PDE 4 inhibitor and/or cyclosporin A, and preferably in the additional presence of forskolin. The invention further provides a method for inducing differentiation of the PGC/PGCLC thus maintained and amplified into oocytes in the presence of BMP and retinoic acid.