Method for inducing differentiation of pluripotent stem cells into germline stem cell-like cells
|Priority claim No.
|SAITOU, Mitinori, etc.
|Title of Invention
|Method for inducing differentiation of pluripotent stem cells into germline stem cell-like cells
Provided is a method for producing spermatogenic stem cell-like cells in vitro from primordial germ cell-like cells derived from isolated pluripotent stem cells, said method comprising (1) a step for co-culturing the primordial germ cell-like cells with gonad somatic cells by suspension culture to give a reconstituted testis, and (2) a step for culturing the reconstituted testis by gas/liquid interfacial culture so as to induce DDX4-positive and PLZF-positive cells in the reconstituted testis. Also provided is a method for producing germline stem cell-like cells, said method comprising dissociating the spermatogenic stem cell-like cells obtained by the aforesaid method from the reconstituted testis and culturing the same under such conditions that germline stem cells can be induced from spermatogenic stem cells.