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Provided is a method for efficiently producing microglia from pluripotent stem cells, comprising steps of (a) co-culturing a pluripotent stem cell with a feeder cell to obtain a blood progenitor cell, (b) co-culturing the obtained blood progenitor cell with a feeder cell in the presence of IL-3 and/or GM-CSF to obtain an embryonic monocyte, and (c) in the presence of M-CSF, co-culturing the obtained embryonic monocyte together with an astrocyte, or culturing the embryonic monocyte using an astrocyte supernatant.