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Provided is a method for producing a genetically diverse regenerated T cell colony via iPS cells, comprising steps of (1) concentrating a T cell colony that has tropism toward a target tissue or antigen from a sampled genetically diverse T cell colony, (2) reprogramming the concentrated T cell colony in step (1) into iPS cells and culturing the iPS cells while maintaining genetic diversity, and (3) producing a genetically diverse regenerated T cell colony from the cultured iPS cells in step (2).