Related Patent Info
METHOD FOR PRODUCING DOPAMINERGIC NEURONS
Inventor | OZAKI Norio, etc. |
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Assignee | NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY |
Title of Invention | METHOD FOR PRODUCING DOPAMINERGIC NEURONS |
Abstract | Provided is a method for efficiently producing dopaminergic neurons within a short period of time from pluripotent stem cells, comprising steps of (1) culturing pluripotent stem cells in the presence of a TGF-β family inhibitor, a GSK3β inhibitor and a BMP inhibitor, (2) suspension-culturing the cells obtained in (1) in the presence of a TGF-β family inhibitor, a GSK3β inhibitor, FGF8 and a hedgehog signal agonist under normal oxygen partial pressure so as to form neurospheres, and (3) collecting cells constituting the neurospheres and inducing the differentiation thereof into dopaminergic neurons. |