Related Patent Info
METHOD FOR PRODUCING DOPAMINERGIC NEURONS
|OZAKI Norio, etc.
|NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
|Title of Invention
|METHOD FOR PRODUCING DOPAMINERGIC NEURONS
Provided is a method for efficiently producing dopaminergic neurons within a short period of time from pluripotent stem cells, comprising steps of (1) culturing pluripotent stem cells in the presence of a TGF-β family inhibitor, a GSK3β inhibitor and a BMP inhibitor, (2) suspension-culturing the cells obtained in (1) in the presence of a TGF-β family inhibitor, a GSK3β inhibitor, FGF8 and a hedgehog signal agonist under normal oxygen partial pressure so as to form neurospheres, and (3) collecting cells constituting the neurospheres and inducing the differentiation thereof into dopaminergic neurons.