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METHOD FOR PREPARING INDUCED DOPAMINERGIC NEURONAL PROGENITOR BY DIRECT REPROGRAMMING

Inventor CHO, Yee Sook etc.
Assignee KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
Title of Invention METHOD FOR PREPARING INDUCED DOPAMINERGIC NEURONAL PROGENITOR BY DIRECT REPROGRAMMING
Abstract

The present invention relates to a method for preparing an induced dopaminergic neuronal progenitor (iDP), and a cell therapeutic agent and a composition for treating or preventing Parkinson's disease, containing the iDP prepared thereby as an active ingredient, the method comprising the steps of: inducing Oct4, Sox2, Klf4 and c-Myc gene expression in adult cells; and directly reprogramming the adult cells into iDPs by treating the cells with sonic hedgehog (SHH) and fibroblast growth factor 8 (FGF8).In addition, the present invention relates to a method for preparing mesencephalic dopaminergic neurons, comprising the steps of: isolating a neural stem cell-like colony (NSC-like colony) by culturing the iDP; and making the isolated colony be a single cell, and culturing the same in a neuronal differentiation medium. Additionally, the present invention relates to a method for treating Parkinson's disease or a method for screening an agent for preventing or treating Parkinson's disease, by using the cell of the present invention. An iDP prepared by the method for preparing an iDP of the present invention has remarkably high efficiency for differentiating into dopaminergic neurons compared with known methods, and since the somatic cells of a patient can be used, there are no side effects such as immunogenicity and no ethical problems, thereby enabling the iDP to be widely used in the relevant fields.