Related Patent Info

Provided is a method for culturing totipotential stem cells. The method comprises: using an artificial synthetic polypeptide as an extracellular matrix, spreading it on the surface of a cell culture dish, then inoculating the totipotential stem cells onto the surface of the aforementioned culture dish, and injecting in a feeding layer-free culture medium, and then culturing and passaging the inoculated totipotential stem cells. The method provided can support the proliferation of the totipotent stem cells and maintain the proportion of Oct4+ cells at above 80% all the time. After culturing for at least 40 days, namely, after passaging six times and cell doubling about 40 times, the cells still greatly express various markers of the totipotent stem cells, and after proliferation culturing, the karyotype still remains consistent.