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CULTURING PLURIPOTENT STEM CELLS

Inventor CHAN, Yun Shen etc.
Assignee AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Title of Invention CULTURING PLURIPOTENT STEM CELLS
Abstract

A method for culturing and maintaining a pluripotent stem cell in an undifferentiated state is provided. The method comprises culturing the pluripotent stem cell in a medium comprising an MEK inhibitor, a GSK3 inhibitor, a dual inhibitor of AMPK and/or BMP signaling and LIF. A cell produced by the method, cell culture medium and a kit for performing the method described is also provided.