Related Patent Info
DIFFERENTIATION OF PLURIPOTENT STEM CELLS TO INTESTINAL MIDGUT ENDODERM CELLS
| Inventor | RIECK, Sebastian, etc. |
|---|---|
| Assignee | JANSSEN BIOTECH, INC. |
| Title of Invention | DIFFERENTIATION OF PLURIPOTENT STEM CELLS TO INTESTINAL MIDGUT ENDODERM CELLS |
| Abstract | Provided is a method for producing a population of intestinal midgut endoderm cells from human pluripotent stem cells. The method may comprise the steps of (a) culturing the human pluripotent stem cells in a first culture medium containing GDF-8 and a GSK3 inhibitor to produce definitive endoderm cells, (b) culturing the definitive endoderm cells in a second culture medium containing ascorbic acid and FGF7 to produce primitive gut tube cells, and (c) culturing the primitive gut tube cells in a third culture medium containing retinoic acid and BMP2 or BMP4 to produce intestinal midgut endoderm cells. |