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Provided is a method for deriving and maintaining a midbrain-like organoid, comprising steps of (a) culturing pluripotent stem cells to obtain neuronal lineage embryoid bodies, (b) culturing the neuronal lineage embryoid bodies from (a) to obtain midbrain regionalized tissues, (c) embedding and culturing the midbrain regionalized tissues from (b) in an extracellular matrix to obtain developing neuroepithelial tissues, and (d) culturing the neuroepithelial tissues from (c) to obtain a midbrain-like organoid.