Related Patent Info
COLONY FORMING MEDIUM AND USE THEREOF
| Inventor | SLUKVIN, Igor, etc. |
|---|---|
| Assignee | CYNATA THERAPEUTICS LIMITED |
| Title of Invention | COLONY FORMING MEDIUM AND USE THEREOF |
| Abstract | Provided is a mesenchymal stem cell (MSC) expressing miR-145-5p, miR-181b-5p and miR-214-3p, but not miR-127-3p or miR-299-5p. Also provided is a method for producing a MSC, comprising steps of (a) culturing a primitive mesoderm cell, which may be differentiated from a pluripotent stem cell, in a mesenchymal-colony forming medium comprising LiCl and FGF2, but excluding PDGF to form a mesenchymal colony and (b) culturing the mesenchymal colony adherently to produce the MSC. |