Related Patent Info
GENERATION OF FUNCTIONAL BETA CELLS FROM HUMAN PLURIPOTENT STEM CELL-DERIVED ENDOCRINE PROGENITORS
| Inventor | CHRISTOPHERSEN, Nicolaj, Strøyer, etc. |
|---|---|
| Assignee | NOVO NORDISK A/S |
| Title of Invention | GENERATION OF FUNCTIONAL BETA CELLS FROM HUMAN PLURIPOTENT STEM CELL-DERIVED ENDOCRINE PROGENITORS |
| Abstract | Provided is a method for generating functional mature beta cells from human pluripotent stem cell-derived endocrine progenitors comprising the steps of (1) culturing the stem cell-derived endocrine progenitor cells in a medium comprising histone methyltransferase EZH2 inhibitor, TGF-beta signaling pathway inhibitor, Heparin and Nicotinamide in basal medium, to obtain INS+ and NKX6.1+ double positive immature beta cells and (2) culturing the beta cells obtained in step (1) with 12% knockout serum replacement and GABA , to obtain functional mature beta cells. |