Related Patent Info
METHODS FOR THE RE-DERIVATION OF DIVERSE PLURIPOTENT STEM CELL-DERIVE BROWN FAT CELLS
| Inventor | WEST, Michael, D., etc. |
|---|---|
| Assignee | BIOTIME, INC. |
| Title of Invention | METHODS FOR THE RE-DERIVATION OF DIVERSE PLURIPOTENT STEM CELL-DERIVE BROWN FAT CELLS |
| Abstract | Provided is a method for producing brown adipocytes comprising steps of; (a) providing a pluripotent stem cell-derived clonal embryonic progenitor cell line that expresses DI03, DLK1, ZIC2, SLC1A3 and SBSN but does not express COX7A1 and does not express one or more of HOXA5, IL13RA2, DLX5, CRABP1, NEFM, PRG4, and RBP1, (b) differentiating the said clonal embryonic progenitor cell line to a brown adipocyte lineage in the presence of a PPARγ agonist, and (c) screening the said differentiated cell line for the expression of UCP1 marker wherein the presence of the UCP1 marker identifies brown adipocytes. |