Related Patent Info
METHODS FOR DIFFERENTIATING CELLS INTO HEPATIC STELLATE CELLS
| Inventor | VERFAILLIE, Cathérine, etc. |
|---|---|
| Assignee | KATHOLIEKE UNIVERSITEIT LEUVEN, etc. |
| Title of Invention | METHODS FOR DIFFERENTIATING CELLS INTO HEPATIC STELLATE CELLS |
| Abstract | Provided is a method for producing cells with a hepatic stellate cell phenotype, comprising steps of; (a) culturing pluripotent cells with about 1 ng/ml to about 200 ng/ml BMP4, (b) culturing the cells of step (a) with about 1 ng/ml to about 200 ng/ml FGF1 and about 1 ng/ml to about 200 ng/ml FGF3, (c)culturing the cells of step (b) with about 0.5 μΜ to about 50 μΜ retinol and about 10 μΜ to about 1 m M palmitic acid, and (d) isolating hepatic stellate cells produced by the differentiation protocol. |