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Provided is a method of culturing cells whereby the cells mimic the structure and function of the blood brain barrier, comprising steps of (a) providing a fluidic device comprising a membrane that comprises a top surface and a bottom surface, (b) seeding cells those may be selected from the group consisting of stem cell-derived cells, cells differentiated from stem cells and primary cells on said bottom surface, and (c) culturing said seeded cells under conditions that support the maturation of brain microvascular endothelial cells. The method may further comprise a step of (d) seeding stem cell-derived cells or cells differentiated from stem cells on said top surface and culturing said top surface seeded cells under conditions that support the maturation of astrocytes or neurons.