Related Patent Info
METHODS OF GENERATING HUMAN INNER EAR SENSORY EPITHELIA AND SENSORY NEURONS
| Inventor | KOEHLER, Karl, R, etc. |
|---|---|
| Assignee | INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION |
| Title of Invention | METHODS OF GENERATING HUMAN INNER EAR SENSORY EPITHELIA AND SENSORY NEURONS |
| Abstract | Provided is a method for obtaining human pre-otic epithelial cells comprising steps of (a) culturing human pluripotent stem cell aggregates in a culture medium comprising a BMP and a TGFβinhibitor for about 8 to 10 days, (b) further culturing the cultured aggregates of (a) in the presence of a FGF and a BMP inhibitor for about 4 days, and (c) contacting the further cultured aggregates of (b) to a Wnt agonist for about 4 days; whereby cells within the contacted aggregates differentiate into pre-otic epithelial cells. |