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METHODS FOR CARDIAC DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS

Inventor YAZAWA, Masayuki, etc.
Assignee THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Title of Invention METHODS FOR CARDIAC DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS
Abstract

Provided is a method for inducing differentiation of monolayer cardiac cells from pluripotent stem cells, comprising steps of (i) culturing the pluripotent stem cells in a medium containing at least two GSK3 inhibitors and (ii) culturing the cells obtained in (i) in a medium containing one or more WNT signaling inhibitor(s).