Related Patent Info
LONG-TERM SELF-RENEWING NEURAL STEM CELLS
Inventor | SCHWAMBORN, Jens, etc. |
---|---|
Assignee | UNIVERSITÉ DU LUXEMBOURG |
Title of Invention | LONG-TERM SELF-RENEWING NEURAL STEM CELLS |
Abstract | Provided is a method for obtaining neural stem cells (NSCs) comprising steps of (i) culturing iPS cells in a medium containing an activin/TGF-β inhibitor, a canonical WNT activator, a BMP inhibitor, and a SHH activator, (ii) cultivating the cells obtained in (i) in a medium containing a canonical WNT activator, a SHH activator, and an antioxidant, and (iii) maintaining the cells obtained in (ii) in a medium containing a FGF activator, an EGF activator, and a LIF activator. The obtained NSCs may be further differentiated into neurons or glial cells, preferably astrocytes. |