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LONG-TERM SELF-RENEWING NEURAL STEM CELLS

Inventor SCHWAMBORN, Jens, etc.
Assignee UNIVERSITÉ DU LUXEMBOURG
Title of Invention LONG-TERM SELF-RENEWING NEURAL STEM CELLS
Abstract

Provided is a method for obtaining neural stem cells (NSCs) comprising steps of (i) culturing iPS cells in a medium containing an activin/TGF-β inhibitor, a canonical WNT activator, a BMP inhibitor, and a SHH activator, (ii) cultivating the cells obtained in (i) in a medium containing a canonical WNT activator, a SHH activator, and an antioxidant, and (iii) maintaining the cells obtained in (ii) in a medium containing a FGF activator, an EGF activator, and a LIF activator.  The obtained NSCs may be further differentiated into neurons or glial cells, preferably astrocytes.