Related Patent Info
A METHOD FOR REGENERATING THE INTERVERTERBRAL DISC WITH NOTOCHORDAL CELLS
| Inventor | GAZIT, Zulma, etc. |
|---|---|
| Assignee | CEDARS-SINAI MEDICAL CENTER |
| Title of Invention | A METHOD FOR REGENERATING THE INTERVERTERBRAL DISC WITH NOTOCHORDAL CELLS |
| Abstract | Provided is a method for generating induced notochordal cells (iNCs) from induced pluripotent stem cells (iPSCs) comprising steps of (i) to (iii) below, and a method for modulating intervertebral disc degeneration of a subject by administering the iNCs to the subject; (i)culturing the iPSCs in the presence of a GSK3 inhibitor to form primitive streak (PS) cells, (ii) contacting the PS cells with a vector encoding Brachyury, and (iii) expressing Brachyury in the PS cells; thereby inducing formation of iNCs. |