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Provided is a method for preparing an organotypic vascularized tissue, skin or mucosa equivalent from mammalian pluripotent stem cells comprising steps of i) culturing the stem cells under conditions to induce the formation of endothelial cells (SC-ECs), vascular smooth muscle cells/pericytes (SC-vSMCs), fibroblasts (SC-Fib) and keratinocytes (SC-KCs), ii) seeding the SC-ECs, SC-vSMCs and, optionally, SC-Fib obtained in step i) in or on a scaffold to induce formation of a vascularized dermal layer, and iii) seeding the SC-KCs obtained in step i) onto the vascularized dermal layer obtained in step ii) to induce the formation of a stratified layer of keratinized epidermis upon said vascularized dermal layer and provide an organotypic vascularized skin or mucosa equivalent.