Patent Portfolio
Method for inducing differentiation of pluripotent stem cells into germline stem cell-like cells
| Ref. No. | AJ188 |
|---|---|
| Appl. No. （filing date） |
PCT/JP2018/021775（2018/6/6） |
| Pub. No. （Pub. date） |
WO2018/225802（2018/12/13） |
| Priority claim No. （Priority date） |
JP2017-113054 (2017/06/07) |
| Patent No. | - |
| Inventor | SAITOU, Mitinori, etc. |
| Assignee | Kyoto University |
| Title of Invention | Method for inducing differentiation of pluripotent stem cells into germline stem cell-like cells |
| Abstract | Provided is a method for producing spermatogenic stem cell-like cells in vitro from primordial germ cell-like cells derived from isolated pluripotent stem cells, said method comprising (1) a step for co-culturing the primordial germ cell-like cells with gonad somatic cells by suspension culture to give a reconstituted testis, and (2) a step for culturing the reconstituted testis by gas/liquid interfacial culture so as to induce DDX4-positive and PLZF-positive cells in the reconstituted testis. Also provided is a method for producing germline stem cell-like cells, said method comprising dissociating the spermatogenic stem cell-like cells obtained by the aforesaid method from the reconstituted testis and culturing the same under such conditions that germline stem cells can be induced from spermatogenic stem cells. |
| Comments |
- |
| Updated date | 2019/6/13 |
| Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2018-225802/CBD7FF0AFC9D1C6E639FBD1F41276E35531FD75874D17D4CC3EF9908329021F6/50/en |