Patent Portfolio
Method for inducing differentiation of airway epithelial cells
| Ref. No. | AJ126 |
|---|---|
| Appl. No. （filing date） |
PCT/JP2016/059786（2016/3/18） |
| Pub. No. （Pub. date） |
WO2016/148307（2016/9/22） |
| Priority claim No. （Priority date） |
JP2015-056791 (2015/3/19) |
| Patent No. | - |
| Inventor | GOTO Shimpei, etc. |
| Assignee | Kyoto University |
| Title of Invention | Method for inducing differentiation of airway epithelial cells |
| Abstract | This invention provides a method for producing an airway epithelial cell from a pluripotent stem cell comprising steps of; (1) culturing a pluripotent stem cell in a medium containing Activin A and a GSK3β inhibitor, (2) culturing the cell obtained in (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor, (3) culturing the cell obtained in (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor, (5) 3D culturing the ventral anterior foregut cell obtained after step (3) in a medium containing a GSK3β inhibitor, FGF10, and a ROCK inhibitor, and (6) 3D culturing the central airway epithelial progenitor cell obtained in (5) in a medium containing a ROCK inhibitor. The method may further comprise the step (4); culturing the ventral anterior foregut cell obtained after step (3) in a medium containing a GSK3β inhibitor and FGF10 to induce an airway epithelial progenitor cell. |
| Comments |
- |
| Updated date | 2016/11/21 |
| Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2016-148307/D140F53CD0514D6C54E5837FB003DADC3C7EC5AE82715616E964076916BF086F/50/en |