Patent Portfolio
mRNA switch, and method for regulating expression of protein using same
| Ref. No. | AJ229 |
|---|---|
| Appl. No. （filing date） |
PCT/JP2020/044905（2020/12/2） |
| Pub. No. （Pub. date） |
WO2021/112136（2021/6/10） |
| Priority claim No. （Priority date） |
JP2019-218386(2019/12/02) |
| Patent No. | - |
| Inventor | SAITO Hirohide, etc. |
| Assignee | Kyoto University |
| Title of Invention | mRNA switch, and method for regulating expression of protein using same |
| Abstract | Provided is a novel mRNA switch which enables the construction of a precise translation regulation circuit and can be used as a translation regulation module having versatility and orthogonality. In one embodiment, the mRNA switch of the invention comprises an artificial mRNA molecule which comprises (i) a nucleic acid sequence capable of being recognized specifically by an input protein comprising Cas protein or a variant thereof and (ii) a nucleic acid sequence encoding an output protein, wherein the nucleic acid sequence (i) is located on the 5'- or 3'-side of the nucleic acid sequence (ii) and the nucleic acid sequences (i) and (ii) are operably linked to each other. According to the invention, a mRNA switch having a translation control module that does not interfere with other modules can be obtained, enabling a wide variety of applications such as safer and efficient cell programming. |
| Comments |
- |
| Information (External link) |
https://www.j-platpat.inpit.go.jp/c1800/PU/WO-A-2021-112136/9EC3510EE263BC819E676CD00847D8AAA660E457330E34916DF355ED6F09617B/50/en |