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Method for producing mature myotube cells from skeletal muscle stem cells

Ref. No. AJ214
Appl. No. (filing date) PCT/JP2020/027658 (2020/07/16)
Pub. No. (Pub. date) WO2021/15086 (2021/01/28)
Priority claim No. (Priority date)

JP2019-134123(2019/07/19)

Patent No.

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Inventor SAKURAI Hidetoshi, etc.
Assignee Kyoto University
Title of Invention Method for producing mature myotube cells from skeletal muscle stem cells
Abstract

Provided is a method for producing mature myotube cells from skeletal muscle stem cells, comprising the steps of: (1) seeding skeletal muscle stem cells in a cell culture container and culturing the cells using a growth medium; (2) exchanging the medium with a differentiation medium and continuing the culture until some of the cells show a spindle shape; and (3) overlaying a gel, which contains an extracellular matrix component, on the cells and performing the culture using the differentiation medium. The mature myotube cells produced according to the invention from a patient with a genetic muscle disease can suitably be used for the pathological study of the disease and for drug screening.

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