Method for producing mature myotube cells from skeletal muscle stem cells
|Appl. No. (filing date)||PCT/JP2020/027658 (2020/07/16)|
|Pub. No. (Pub. date)||WO2021/15086 (2021/01/28)|
|Priority claim No. (Priority date)||
|Inventor||SAKURAI Hidetoshi, etc.|
|Title of Invention||Method for producing mature myotube cells from skeletal muscle stem cells|
Provided is a method for producing mature myotube cells from skeletal muscle stem cells, comprising the steps of: (1) seeding skeletal muscle stem cells in a cell culture container and culturing the cells using a growth medium; (2) exchanging the medium with a differentiation medium and continuing the culture until some of the cells show a spindle shape; and (3) overlaying a gel, which contains an extracellular matrix component, on the cells and performing the culture using the differentiation medium. The mature myotube cells produced according to the invention from a patient with a genetic muscle disease can suitably be used for the pathological study of the disease and for drug screening.