Method for producing nephron progenitor cells
|Appl. No. (filing date)||PCT/JP2020/016969 (2020/04/17)|
|Pub. No. (Pub. date)||WO2020/213734 (2020/10/22)|
|Priority claim No. (Priority date)||
|Inventor||OSAFUNE Kenji, etc.|
|Title of Invention||Method for producing nephron progenitor cells|
This invention relates to an efficient and stable method for producing nephron precursors or nephron organoids through a stepwise differentiation of pluripotent stem cells. More specifically, the invention is characterized by including the following two novel methods: (1) a method for producing late presomitic mesoderm cells, comprising a step of culturing presomitic mesoderm cells in a medium containing a specified set of growth factors and small molecule inhibitors; and (2) a method for producing posterior intermediate mesoderm cells, comprising a step of culturing late presomitic mesoderm cells in a medium containing a specified set of growth factors.