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Vector and gene introduction agent for monitoring and visualizing cell differentiation using expression of micro RNA as indicator and monitoring and visualizing method using thereof

Ref. No. AJ161
Appl. No. (filing date) US 15/697,831 (2017/09/07)
Pub. No. (Pub. date) US 2019-0071736 (2019/03/07)
Priority claim No. (Priority date)

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Patent No.

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Inventor SAITO Hirohide, etc.
Assignee Kyoto University
Title of Invention Vector and gene introduction agent for monitoring and visualizing cell differentiation using expression of micro RNA as indicator and monitoring and visualizing method using thereof
Abstract

This invention provides a transposon-based vector as well as an episomal vector-based gene introduction agent, both of which are featured in a reporter gene sequence comprising an inducible promoter, a sequence encoding a first marker protein, a mRNA stabilizing sequence, a miRNA target sequence, and a sequence encoding a second marker protein, thereby enabling a continuous visualization of cell differentiation status by using expression of a miRNA as an indicator.

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Updated date 2019/07/19
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