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Gene mutation introduction method

Ref. No. AJ185
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JP2017-139268 (2017/07/18)

Patent No.


Inventor HOTTA, Akitsu, etc.
Assignee Kyoto University
Title of Invention Gene mutation introduction method

Provided is a gene mutation introduction method with improved sequence-specificity comprising a step of expressing a fusion protein comprising a sequence-specific DNA cleavage enzyme and a nuclear receptor under control of an expression-inducing promoter, a step of forming a double-strand DNA break in genomic DNA in a sequence-specific manner by transferring the fusion protein into nucleus, and a step of repairing the double-strand DNA break by utilizing a DNA break repair mechanism and thereby introducing a gene mutation into the vicinity of the double-strand DNA break.



Updated date 2019/6/13
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